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rab7 d95f2 rabbit mab (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc rab7 d95f2 rabbit mab

RSV entry into cells triggers actin polymerization and endosome formation by cholesterol-rich lipid rafts. ( A ) RSV enters cells by actin-mediated endocytosis. HEp-2 or A549 cells were infected with RSV A2 (MOI = 30) (90 min, 37°C) and fixed (4% PFA, 15 min, room temperature). Cell nuclei were labeled with DAPI (cyan), lipid rafts with Alexa 647-CTB (blue), cholesterol with NBD-cholesterol (green), and F-actin with CellMask orange actin tracking dye (yellow), and images were acquired using confocal microscopy. Scale bar = 10 µm. Intensity profiles show colocalization along the direction of the yellow line. Pearson’s correlation between lipid rafts, cholesterol or F-actin, and RSV. n = 16 cells per group. Error bars represent a 95% confidence interval. ( B ) RSV trafficking from early to late endosomes. HEp-2 or A549 cells were infected with RSV A2 (MOI = 30) (150 min, 37°C) and fixed. Cell nuclei were labeled with DAPI (cyan), lipid rafts with Alexa 647-CTB (blue), and cholesterol with NBD-cholesterol (green). Immunodetection of RSV F (blue), RSV-FITC (green), EEA1, <t>Rab7,</t> and STX 6 (red) was performed using confocal microscopy. Scale bar = 10 µm. Intensity profiles show colocalization along the direction of the yellow line. Pearson’s correlation between lipid rafts, cholesterol or F-actin, and RSV. n = 16 cells per group. Error bars represent a 95% confidence interval.

Rab7 D95f2 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+rab7+d95f2/pmc12403703-202-16-36
Average 86 stars, based on 1 article reviews

rab7 d95f2 rabbit mab - by Bioz Stars, 2026-09

86/100 stars

Images

1) Product Images from "Cholesterol-rich lipid rafts mediate endocytosis as a common pathway for respiratory syncytial virus entry into different host cells"

Article Title: Cholesterol-rich lipid rafts mediate endocytosis as a common pathway for respiratory syncytial virus entry into different host cells

Journal: Microbiology Spectrum

doi: 10.1128/spectrum.01192-25

Figure Legend Snippet: RSV entry into cells triggers actin polymerization and endosome formation by cholesterol-rich lipid rafts. ( A ) RSV enters cells by actin-mediated endocytosis. HEp-2 or A549 cells were infected with RSV A2 (MOI = 30) (90 min, 37°C) and fixed (4% PFA, 15 min, room temperature). Cell nuclei were labeled with DAPI (cyan), lipid rafts with Alexa 647-CTB (blue), cholesterol with NBD-cholesterol (green), and F-actin with CellMask orange actin tracking dye (yellow), and images were acquired using confocal microscopy. Scale bar = 10 µm. Intensity profiles show colocalization along the direction of the yellow line. Pearson’s correlation between lipid rafts, cholesterol or F-actin, and RSV. n = 16 cells per group. Error bars represent a 95% confidence interval. ( B ) RSV trafficking from early to late endosomes. HEp-2 or A549 cells were infected with RSV A2 (MOI = 30) (150 min, 37°C) and fixed. Cell nuclei were labeled with DAPI (cyan), lipid rafts with Alexa 647-CTB (blue), and cholesterol with NBD-cholesterol (green). Immunodetection of RSV F (blue), RSV-FITC (green), EEA1, Rab7, and STX 6 (red) was performed using confocal microscopy. Scale bar = 10 µm. Intensity profiles show colocalization along the direction of the yellow line. Pearson’s correlation between lipid rafts, cholesterol or F-actin, and RSV. n = 16 cells per group. Error bars represent a 95% confidence interval.

Techniques Used: Infection, Labeling, Confocal Microscopy, Immunodetection

Microscopy:

Article Title: Cholesterol and ORP1L-mediated ER contact sites control autophagosome transport and fusion with the endocytic pathway.
Article Snippet: Puromycin (Gibco) was used at 4 mgml 1. .. Antibodies used: rabbit anti-LC3B (Novus Biologicals NB100-2220, used for all experiments except costaining with endogenous Rab7, microscopy 1:400, western blot 1:1,000), mouse anti-LC3B (Cosmo Bio LC3-1703, only used for co-staining with Rab7, 1:100), rabbit anti-ORP1L (1:1,000), rabbit anti-GFP (1:1,000), mouse anti-CD63 (1:500) and rabbit anti-CD63 (1:100) (all described in ref. 33), rabbit anti-PDI (1:10, as described in ref. 67), mouse anti-HA (Covance 16B12, 1:1,000), rat anti-HA 3F10 (Roche, 1:200), mouse anti-p62 D3 (1:500), goat anti-calnexin C-20 (1:40), goat anti-VAP-A K15 (microscopy 1:40, western blot 1:500), mouse anti-Ubiquitin P4D1 (1:50) (all Santa Cruz), rabbit anti-Rab7 D95F2 (Cell Signaling Technologies, 1:100), and mouse anti-p150glued (BD Biosciences 610473, 1:100), rabbit anti-Flag F7425 and mouse anti-Flag M2 (Sigma, 1:1,000). .. Secondary antibodies (goat anti-mouse Alexa 405/488/568/647, goat anti-rabbit Alexa 488/568/647, donkey anti-goat Alexa 488/568, donkey anti-mouse Alexa 488/555/647 and donkey anti-rabbit Alexa 488/647) were purchased from Life Technologies and donkey anti-rat CF568 from Biotium (all 1:200).

Western Blot:

other:

Article Title: Molecular determinants of the crosstalk between endosomal microautophagy and chaperone-mediated autophagy
Article Snippet: All secondary antibodies for routine immunofluorescence were from ThermoFisher Scientific.

Article Title: ABMA, a small molecule that inhibits intracellular toxins and pathogens by interfering with late endosomal compartments
Article Snippet: The following commercial antibodies were used in this study: rabbit anti-Rab7 (ab137029) were from Abcam; Rabbit anti-Rab7 (D95F2) (#9367), rabbit anti-EEA1 (#3288) were from Cell Signaling, Rabbit anti-Lamp1 (L1418) and mouse anti-beta-actin (A2228) from Sigma-Aldrich; rabbit anti-MEK2 (N-20, sc-524), mouse anti-chlamydial trachomatis HSP60 protein (clone A57-B9, sc-57840) from Santa Cruz Technologies; mouse anti-SNAP-25 (SMI-81, 836304) from Biolegend; mouse anti-RNP (ribonucleoprotein) conjugated with FITC from Fujirebio; Alexa 488-donkey anti-rabbit (A10042), Alexa 546-donkey anti-mouse (A10036) and LysoTracker Deep Red (L12492) were from ThermoFisher Scientific.

Article Title: Molecular determinants of the crosstalk between endosomal microautophagy and chaperone-mediated autophagy
Article Snippet: rabbit anti-Rab7 (D95F2) , Cell Signaling , Cat # 9367; RRID:AB_1904103.

Article Title: E-Cadherin Is a Structuring Component of Invadopodia in Pancreatic Cancer.
Article Snippet: Mouse anti- E- cadherin (24E10), rabbit anti- P- cadherin (21300S) and rabbit anti- Rab7 (D95F2) were from Cell Signalling.

Article Title: Loss of the proton-activated chloride channel in neurons impairs AMPA receptor endocytosis and LTD via endosomal hyper-acidification
Article Snippet: Rabbit anti-Rab7 (D95F2) , Cell Signaling Technology , Cat. 9367, RRID: AB_1904103.

Immunofluorescence:

Article Title: The small GTPase RAB10 regulates endosomal recycling of the LDL receptor and transferrin receptor in hepatocytes
Article Snippet: Rabbit anti GM130 , Abcam/ab52649 , Immunofluorescence , 1:100. .. Rabbit anti Rab7(D95F2) , Cell Signaling Technology, Inc/ 9367T , Immunofluorescence , 1:100. .. Donkey anti-Sheep IgG-Alexa Fluor 488 , Thermo Scientific / A-11015 , Immunofluorescence , 1:500.

Staining:

Article Title: ELD607 specifically traffics Orai1 to the lysosome leading to inhibition of store operated calcium entry.
Article Snippet: Cells were then fixed in 4% paraformaldehyde (PFA) for 15 min at room temperature and permeabilized in 0.1% tritonX100 (Millipore-Sigma, T8787) in PBS for 10 min at room temperature. .. Cells were then blocked at room temperature for 2 h and stained with one of the following primary antibodies - rabbit anti-LAMP1 D2D11 (#9091, 1:500, Cell Signaling Technologies), rabbit anti-Rab7 D95F2 (#9367, 1:200, Cell Signaling Technologies), rabbit anti-Rab11 D4F5 (#5589, 1:200, Cell Signaling Technologies), rabbit anti-Rab5A (#2143, 1:200, Cell Signaling Technologies), mouse anti-Ubiquitin P4D1 (#3936, 1:200, Cell Signaling Technologies), rabbit anti-Orai1 A.S. Goriounova et al. .. Cell Calcium 123 (2024) 102945 (SAB3500412, 1:100, Millipore-Sigma) overnight at 4 ◦C in the blocking solution.

Image Search Results

Journal: Microbiology Spectrum

Article Title: Cholesterol-rich lipid rafts mediate endocytosis as a common pathway for respiratory syncytial virus entry into different host cells

doi: 10.1128/spectrum.01192-25

Figure Lengend Snippet: RSV entry into cells triggers actin polymerization and endosome formation by cholesterol-rich lipid rafts. ( A ) RSV enters cells by actin-mediated endocytosis. HEp-2 or A549 cells were infected with RSV A2 (MOI = 30) (90 min, 37°C) and fixed (4% PFA, 15 min, room temperature). Cell nuclei were labeled with DAPI (cyan), lipid rafts with Alexa 647-CTB (blue), cholesterol with NBD-cholesterol (green), and F-actin with CellMask orange actin tracking dye (yellow), and images were acquired using confocal microscopy. Scale bar = 10 µm. Intensity profiles show colocalization along the direction of the yellow line. Pearson’s correlation between lipid rafts, cholesterol or F-actin, and RSV. n = 16 cells per group. Error bars represent a 95% confidence interval. ( B ) RSV trafficking from early to late endosomes. HEp-2 or A549 cells were infected with RSV A2 (MOI = 30) (150 min, 37°C) and fixed. Cell nuclei were labeled with DAPI (cyan), lipid rafts with Alexa 647-CTB (blue), and cholesterol with NBD-cholesterol (green). Immunodetection of RSV F (blue), RSV-FITC (green), EEA1, Rab7, and STX 6 (red) was performed using confocal microscopy. Scale bar = 10 µm. Intensity profiles show colocalization along the direction of the yellow line. Pearson’s correlation between lipid rafts, cholesterol or F-actin, and RSV. n = 16 cells per group. Error bars represent a 95% confidence interval.

Article Snippet: The Flotillin-1 (D2V7J) rabbit mAb (#18,934T), caveolin-1 (D46G3) rabbit mAb (#3267), EEA1 (C45B10) rabbit mAb (#3288), Rab7 (D95F2) rabbit mAb (#9367), Rab7 (E9O7E) mouse mAb (#95746), and syntaxin 6 (C34B2) rabbit mAb (#2869) were purchased from Cell Signaling Technology.

Techniques: Infection, Labeling, Confocal Microscopy, Immunodetection

Figure 7. AMPK activation downregulates Rab5, Rab7, and TXNIP rapidly. Confocal microscopy of Rab5 (magenta), Rab7 (magenta), and TXNIP (magenta) after SA (A,C), AMPK (A), ULK activator (A), and cycloheximide (B) treatment. (C) Analyses of the time-dependent downregulation of Rab5, Rab7, and TXNIP. Scale bars: 20 µm.

Journal: Cells

Article Title: AMPK Activation Downregulates TXNIP, Rab5, and Rab7 Within Minutes, Thereby Inhibiting the Endocytosis-Mediated Entry of Human Pathogenic Viruses.

doi: 10.3390/cells14050334

Figure Lengend Snippet: Figure 7. AMPK activation downregulates Rab5, Rab7, and TXNIP rapidly. Confocal microscopy of Rab5 (magenta), Rab7 (magenta), and TXNIP (magenta) after SA (A,C), AMPK (A), ULK activator (A), and cycloheximide (B) treatment. (C) Analyses of the time-dependent downregulation of Rab5, Rab7, and TXNIP. Scale bars: 20 µm.

Article Snippet: Antibodies: Anti-PVR (CD155) Antibody, clone 4B3, ZooMab® Rabbit Monoclonal recombinant, Cells 2025, 14, 334 4 of 22 Sigma Aldrich; anti-LDL Receptor Antibody, clone 2N19, ZooMab® Rabbit Monoclonal recombinant, Sigma Aldrich (Taufkirchen, Germany); anti-Rab5A Antibody #2143, Cell Signaling; anti-Rab7 (D95F2) XP® Rabbit mAb #9367, Cell Signaling; and anti-TXNIP Antibody, clone 1K14 ZooMAb® Rabbit Monoclonal, Sigma Aldrich; anti-β-Actin Antibody, clone 6L12, ZooMAb® Rabbit Monoclonal, Sigma Aldrich; anti-SARS-CoV-2 Spike Protein (S1-NTD) Antibody #56996, Cell Signaling, anti-SARS-CoV/SARS-CoV-2 NSP8 Monoclonal Antibody (5A10), Invitrogen; and anti-LAMP1 (D4O1S) Mouse mAb #15665, Cell Signaling; anti-GRP78 Polyclonal Antibody, Invitrogen.

Techniques: Activation Assay, Confocal Microscopy

iPS-cell derived podocytes have reduced expression of slit diaphragm proteins, ER congestion, and reduced lysosomes. Panel A shows representative immunoblots of podocyte markers including Nephrin, Synaptopodin, TRPC6, Podocin, CD2AP, GLEPP1, and WT1, the housekeeping protein GAPDH. Panel B shows the quantification of immunoblots for the podocyte markers. Data represents three biologically independent experiments and is defined as ± standard error of the mean. We performed one-way ANOVA with Dunnett’s multiple comparison post hoc analyses with a family-wise alpha threshold of 0.05. Panel C shows electron micrograph of differentiated podocytes with protein aggresomes in the ER of Duke 5 and Duke 7 podocytes. Panel D shows LAMP1 vesicles, Podocin, and TRPC6 expression in podocytes. The LAMP1 vesicle number remains unchanged in the podocytes. Panel E shows lysosomal biogenesis-associated RAB7 expression in the podocytes. RAB7 expression was reduced in the mutants. Data is representative of three biologically independent experiments and the data is defined as ± standard error of the mean. We performed one-way ANOVA with Sidak’s multiple comparison post hoc analyses with a family-wise alpha threshold of 0.05. Scale bar: Panel C, top row 1000 µm, bottom row 500 nm; Panel D, top row 20 µm, bottom row 5 µm; Panel E, 100 µm.

Journal: bioRxiv

Article Title: A Novel TRPC6 Mutation Causes Autosomal Dominant FSGS

doi: 10.1101/2025.02.11.637765

Figure Lengend Snippet: iPS-cell derived podocytes have reduced expression of slit diaphragm proteins, ER congestion, and reduced lysosomes. Panel A shows representative immunoblots of podocyte markers including Nephrin, Synaptopodin, TRPC6, Podocin, CD2AP, GLEPP1, and WT1, the housekeeping protein GAPDH. Panel B shows the quantification of immunoblots for the podocyte markers. Data represents three biologically independent experiments and is defined as ± standard error of the mean. We performed one-way ANOVA with Dunnett’s multiple comparison post hoc analyses with a family-wise alpha threshold of 0.05. Panel C shows electron micrograph of differentiated podocytes with protein aggresomes in the ER of Duke 5 and Duke 7 podocytes. Panel D shows LAMP1 vesicles, Podocin, and TRPC6 expression in podocytes. The LAMP1 vesicle number remains unchanged in the podocytes. Panel E shows lysosomal biogenesis-associated RAB7 expression in the podocytes. RAB7 expression was reduced in the mutants. Data is representative of three biologically independent experiments and the data is defined as ± standard error of the mean. We performed one-way ANOVA with Sidak’s multiple comparison post hoc analyses with a family-wise alpha threshold of 0.05. Scale bar: Panel C, top row 1000 µm, bottom row 500 nm; Panel D, top row 20 µm, bottom row 5 µm; Panel E, 100 µm.

Article Snippet: The primary antibodies used included guinea pig Anti-Nephrin (ARP; #GPN02, 1:500), rabbit Anti-Podocin (Abcam, mAb #ab50339, 1:1000), rabbit Anti-TRPC6 (Abcam, pAb #ab228771) and mouse Anti-CD2AP (B-4) (Santa Cruz, #sc-25272), Calnexin (C5C9) Anti-Rabbit (Cell Signaling, mAb #2679), LAMP1 (D2D11) XP Anti-Rabbit (Cell Signaling, mAb #9091), RAB7 (D95F2) XP and Anti-Rabbit mAb (Cell Signaling, mAb #9367).

Techniques: Derivative Assay, Expressing, Western Blot, Comparison

Journal: Current Biology

Article Title: Systems mapping of bidirectional endosomal transport through the crowded cell

doi: 10.1016/j.cub.2024.08.026

Figure Lengend Snippet:

Article Snippet: Rabbit monoclonal anti-Rab7 (D95F2) , Cell signaling Technology , Cat#9367; RRID: AB_1904103.

Techniques: Recombinant, CRISPR, Sequencing, Introduce, Cloning, Software